So I just read Friendship is Magic #2, and there was something that was too much of an incredible coincidence not to be a reference to BlackGryph0n's Applelicious video.
@mashuga31A spider colored like Derpy Hooves, with bubbles on its back, steals applejacks hat and says "I'm a cowboy! Howdy howdy howdy!" Referencing toy story and what was the top comment on the video for a while.